Class A - Animals will suffer no pain or distress greater than produced by routine injections or venipuncture. Procedures included are injections, tatooing, blood sampling, chemical restraint with sedatives to ease handling, and terminal harvesting of tissues from animals with no surgical manipulations.

Class B1 - Nonsurvival surgical procedures will be performed with appropriate anesthesia. Analgesics and/or tranquilizers should be also be considered according to the procedure planned.

Class B2 - Survival surgery procedures will be performed with appropriate anesthesia. Analgesics and/or tranquilizers should also be considered according to the procedure planned.

Class C - Animals may experience pain or distress greater than that produced by routine injections or venipucture and might not receive anesthetics, analgesics or tranquilizers since this will adversely affect the study. Includes transplantable tumors, in vivo hybridoma work and most drug studies.

Class D - Animals will be restrained longer than 4 hours without analgesics, anesthetics or tranquilizers.

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USDA Reporting Categories:

Column B - Animals being bred, conditioned, or held for use in teaching, testing, experiments, research, or surgery but not yet used for such purposes.

Column C - Animals upon which teaching, research, experiments, or tests were conducted involving no pain, distress, or use of pain-relieving drugs.

Column D - Animals upon which experiments, teaching, research, surgery, or tests were conducted involving accompanying pain or distress to the animals and for which appropriate anesthetic, analgesic, or tranquilizing drugs were used.

Column E - Animals upon which teaching, experiments, research, surgery, or tests were conducted involving accompanying pain or distress to the animals and for which the use of appropriate anesthetic, analgesic, or tranquilizing drugs would have adversely affected the procedures, results, or interpretation of the teaching, research, experiments, surgery, or tests. (An explanation of the procedures producing pain or distress in these animals and the reasons such drugs were not used must be attached to the USDA annual report.)

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For additional information, see the following:

• General Instructions
• Application Explanation
• Progress Report Explanation
• Review Process

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