<Department of Microbiology & Immunology, UT Health Science Center at San Antonio Double Helix rotating/>

Sequencing


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Turnaround Time
Service & Fees

Discounts
Submission Guidelines
Template Preparation
Reaction Requirements
Getting Samples to NAC
What to Expect



Turnaround Time




Standard Sequencing
Sequencing runs are done 3-4 times a week with projects processed on a first come, first serve basis. All samples that are submitted by 11:00am will be processed the same day with results available the next working day in most cases. When workload exceeds our maximum capacity, the turnaround times may vary between 1-3 days and users will be notified of the extended turnaround time.

HT and R2R These sequencing projects allow for expedited workflows prior to loading on a sequencer, so they will have priority over full service standard sequencing orders. Turnaround time can be next day but will vary between one and three days depending on volume.


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Service & Fees


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Discounts



Discounted pricing is available for HT (high-throughput) and also R2R (ready to run) style sequencing . These discounts are volume-based and involve a monthly time component during which the sequencing must be submitted.




  • The tiered discounts do not fit my overall project; are there any others?

    Further discounting on high volume work or projects with custom services can be negotiated and given on a quote basis. Please inquire with the NAC director to discuss your project or for more information.


  • Salary support for NAC staff.

    For large projects, reduced pricing can also be achieved through salary support for our staff. Pricing is negotiated in advance and usually will require % effort on a grant or some other mechanism for cost recovery. In some cases, shared instrumentation that advances the Core's capabilities or research expertise may be substituted for % effort.
    Prices are equivalent to RTR pricing. Custom pricing for volume that can approach "cost" sequencing (what it costs us to sequence) may be negotiated depending on the level of salary support. Please inquire with the NAC director for more information.
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    Submission Guidelines



    Template Preparation:

    * Quality templates will result in quality data. Purity and concentration of templates are crucial factors in sequencing success.

    Reaction Requirements



    • HT Reactions
    •  

      Template Type

      Amount

      Max Volume

      Plasmid

      500ng / rxn

      5.0 ul

      PCR Product

      50ng / rxn

      5.0 u l

      Primers

      2.0pmol / rxn

      2.0 ul

      Total Submission Volume:

      7.0 ul


      *When ordering online please review the HT Submission Guide for further details.
    • R2R Reactions

      *Must be ordered online through the ANAC iLab site.
      Please refer to the R2R Submission Guide for details.

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    Delivering Samples to the NAC



    Main Campus:

    • Bring a printed online form along with your samples to the NAC located in the Basic Science Building Rm 4.059V.


    • After hours or if NAC staff is not available, use the Sequencing Drop Box located in the refrigerator across from the lab. Please enclose your order form and samples in a large NAC sequencing bag, which may be found in the Sequencing Drop Box.


    Research Park

    • Enclose a printed online order form along with your samples in a 9"X12" envelope labeled:
        Nucleic Acids Core Facility
        Dept of Microbiology Rm 4.059 V

    • Send through campus mail. Keep in mind that campus mail is usually only delivered once daily. Inquire with HSC mail services for drop off locations and delivery schedules at 7-5992.
      * R2R users should deliver using the below method or hand deliver to the lab. Campus mail is not recommended as HiDi Formamide is considered a hazardous chemical. If limited to campus mail, please call the NAC for specific instructions regarding allowable plate prep.

    North Campus Users
    • Drop off a printed online form along with your samples to the STRF drop box located in room 258 by 11a.m.

    • NAC staff will make a daily drop off/pick up between 11:00a.m. -11:15a.m.

    Non-UTHSCSA

    • Use a small disposable cooler and disposable ice pack and enclose samples.
    • Place a protected online form (in a plastic bag) inside the cooler with the samples.
    • Use a carrier of choice and send to:
      UTHSCSA
      Attention: Nucleic Acid Core Facility
      Dept of Microbiology Rm 4.059 V
      7703 Floyd Curl Dr.
      San Antonio, TX 78229-3900

      *Please allow the appropriate time for selected shipping method. You may check receipt of your samples online by logging into the NAC GeneSifter site and navigating to the View Orders page.

    What to Expect



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